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- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Potassium channel subfamily K member 18,KCNK18
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Potassium channel subfamily K member 18, KCNK18 ELISA KIT
Product Name:Human Potassium channel subfamily K member 18, KCNK18 ELISA KIT
Packing:96T
Catalog No.:ELI-28088h
Gene Name:KCNK18
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Potassium channel subfamily K member 18(KCNK18
Alternative Name:KCNK18,Potassium channel subfamily K member 18(KCNK18
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Potassium channel subfamily K member 18, KCNK18 ELISA KIT allows for the in vitro quantitative determination of Potassium channel subfamily K member 18(KCNK18 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Potassium channel subfamily K member 18, KCNK18 ELISA KIT has been pre-coated with an Potassium channel subfamily K member 18(KCNK18 antibody specific to Potassium channel subfamily K member 18(KCNK18 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Potassium channel subfamily K member 18(KCNK18 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Potassium channel subfamily K member 18(KCNK18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Potassium channel subfamily K member 18(KCNK18 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Characterization of Calcium Channel Binding
.P., Fox, M., Rees, M. and Perez‐Reyes, E. 1998. Cloning and characterization of alpha1H from human heart, a member of the T‐type Ca2+ channel gene family. Circ. Res. 83:103‐109.
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有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
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