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- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Glutamate receptor 1,GRIA1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Glutamate receptor 1, Gria1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Gria1,GluR1,GLUH1; GLURA; GluA1; HBGR1; GluR-A ,AMPA 1; gluR-1; gluR-K1; glutamate receptor 1; glutamate receptor ionotropic AMPA 1,Glutamate receptor 1GluR-1,Glutamate receptor ionotropic, AMPA-selective glutamate receptor 1;
Search name
Human Gria1 ELISA KIT ,Human GluR1 ELISA KIT ,Human GLUH1 ELISA KIT ,Human GLURA ELISA KIT ,Human GluA1 ELISA KIT ,Human HBGR1 ELISA KIT ,Human GluR-A ELISA KIT ,Human AMPA 1 ELISA KIT ,Human gluR-1 ELISA KIT ,Human gluR-K1 ELISA KIT ,Human glutamate receptor 1 ELISA KIT ,Human glutamate receptor ionotropic AMPA 1 ELISA KIT ,Human Glutamate receptor 1GluR-1 ELISA KIT ,Human Glutamate receptor ionotropic ELISA KIT ,Human AMPA-selective glutamate receptor 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human glutamate receptor 1,GluR-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GluR-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GluR-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GluR-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GluR-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验and, vice versa, insulin receptor crossreacts with IGF-1. Numerous studies suggest that IGF-1-R is very important for mitogenesis and is essential for phenotype transformation, at least in rodents (1). In particular, the IGF-1-R has been described in human breast
Aspects of CNS Lupus: Mouse Models of Anti-NMDA Receptor Antibody Mediated Reactivity
of interest binds the N -methyl-d -aspartate receptor (NMDAR), a receptor for glutamate that is a major excitatory neurotransmitter in the brain involved in synaptic plasticity, in memory and learning, and in emotional responses. We introduce basic
调至 1μg/μl。 4. ELISA 检测:按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数,结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理,全程低温操作,裂解液现配现用; 2. 蛋白定量时标准品与样本同步孵育,稀释采用倍比稀释减少误差; 3. ELISA 实验需每次重绘标准曲线,洗板充分、严格把控显色与终止时间。 免疫检测试剂盒: 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
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