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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Histone deacetylase 5,HDAC5
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Histone deacetylase 5, HDAC5 ELISA KIT
Product Name:Human Histone deacetylase 5, HDAC5 ELISA KIT
Packing:96T
Catalog No.:ELI-27739h
Gene Name:HDAC5
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Histone deacetylase 5(HDAC5
Alternative Name:HDAC5,Histone deacetylase 5(HDAC5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Histone deacetylase 5, HDAC5 ELISA KIT allows for the in vitro quantitative determination of Histone deacetylase 5(HDAC5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Histone deacetylase 5, HDAC5 ELISA KIT has been pre-coated with an Histone deacetylase 5(HDAC5 antibody specific to Histone deacetylase 5(HDAC5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Histone deacetylase 5(HDAC5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Histone deacetylase 5(HDAC5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Histone deacetylase 5(HDAC5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Use of Polyamine Derivatives as Selective Histone Deacetylase Inhibitors
by promoting the reexpression of aberrantly silenced genes that code for tumor suppressor factors. It is unclear which of the 11 histone deacetylase isoforms are important in human cancer. We have designed a series of polyaminohydroxamic acid (PAHA
HDAC(Histone Deacetylase)组蛋白去乙酰化酶活性测定及药物筛选方案
DAC(Histone Deacetylase)组蛋白去乙酰化酶活性测定及药物筛选方组蛋白去乙酰化酶 (histone deacetylase,HDAC )是一类蛋白酶,对染色体的结构修饰和基因表达调控发挥着重要的作用。一般情况下,组蛋白的乙酰化有利于DNA与组蛋白八聚体的解离,核小体结构松弛,从而使各种转录因子和协同转录因子能与DNA结合位点特异性结合,激活基因的转录。在细胞核内,组蛋白乙酰化与组蛋白去乙酰化过程处于动态平衡,并由组蛋白乙酰化转移 酶(histone
, A.D., Rossi, J.J. and Morris, K.V. (2005) The antisense strand of small interfering RNAs directs histone methylation and transcriptional gene silencing in human cells, RNA, 12(2): 256-262.
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