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- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Alpha-(1,3)-fucosyltransferase,FUT4
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Alpha- (1,3)- fucosyltransferase, FUT4 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CD15,FUT4,ELFT; FCT3A; FUC-TIV; FUTIV; LeX; SSEA-1; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; Lewis X; fucT-IV; fucosyltransferase IV; galactoside 3-L-fucosyltransferase; stage-specific embryonic antigen 1; fucosyltransferase 4,Rfuc-t,alpha (1,3) fucosyltransferase, myeloid-specific, alpha-(1,3)-fucosyltransferase 4;
Search name
Human CD15 ELISA KIT ,Human FUT4 ELISA KIT ,Human ELFT ELISA KIT ,Human FCT3A ELISA KIT ,Human FUC-TIV ELISA KIT ,Human FUTIV ELISA KIT ,Human LeX ELISA KIT ,Human SSEA-1 ELISA KIT ,Human ELAM ligand fucosyltransferase ELISA KIT ,Human ELAM-1 ligand fucosyltransferase ELISA KIT ,Human Lewis X ELISA KIT ,Human fucT-IV ELISA KIT ,Human fucosyltransferase IV ELISA KIT ,Human galactoside 3-L-fucosyltransferase ELISA KIT ,Human stage-specific embryonic antigen 1 ELISA KIT ,Human fucosyltransferase 4 ELISA KIT ,Human Rfuc-t ELISA KIT,Human alpha (1,3) fucosyltransferase myeloid-specific ELISA KIT, Human alpha-(1,3)-fucosyltransferase 4 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Alpha- (1,3)- fucosyltransferase, FUT4 beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Alpha- (1,3)- fucosyltransferase, FUT4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human Alpha- (1,3)- fucosyltransferase, FUT4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Alpha- (1,3)- fucosyltransferase, FUT4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Alpha- (1,3)- fucosyltransferase, FUT4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
调至 1μg/μl。 4. ELISA 检测:按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数,结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理,全程低温操作,裂解液现配现用; 2. 蛋白定量时标准品与样本同步孵育,稀释采用倍比稀释减少误差; 3. ELISA 实验需每次重绘标准曲线,洗板充分、严格把控显色与终止时间。 免疫检测试剂盒: 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
操作条件、人员一致 边缘效应 蒸发/酶标板叠放 孵育加封板膜或盖板;避免叠放 孵育温度不均 校准温育箱;避开通风口、窗边等环境波动区 洗板不彻底 确保洗板仪正常;不减少洗液量与次数 注意!!! ELISA试剂盒11月活动即将来袭~敬请期待~ 部分热卖产品推荐 产品货号 产品名称 检测范围 灵敏度 97109ES Human IL18 ELISA Kit 125
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