- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-27227h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Inositol-trisphosphate 3-kinase A,ITPKA
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Inositol- trisphosphate 3- kinase A, ITPKA ELISA KIT
Product Name:Human Inositol- trisphosphate 3- kinase A, ITPKA ELISA KIT
Packing:96T
Catalog No.:ELI-27227h
Gene Name:ITPKA
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Inositol-trisphosphate 3-kinase A(ITPKA
Alternative Name:ITPKA,Inositol-trisphosphate 3-kinase A(ITPKA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Inositol- trisphosphate 3- kinase A, ITPKA ELISA KIT allows for the in vitro quantitative determination of Inositol-trisphosphate 3-kinase A(ITPKA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Inositol- trisphosphate 3- kinase A, ITPKA ELISA KIT has been pre-coated with an Inositol-trisphosphate 3-kinase A(ITPKA antibody specific to Inositol-trisphosphate 3-kinase A(ITPKA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Inositol-trisphosphate 3-kinase A(ITPKA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Inositol-trisphosphate 3-kinase A(ITPKA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Inositol-trisphosphate 3-kinase A(ITPKA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
, Ku. Ku is composed of 70-kDa and approx 80-kDa subunits (called Ku70 and Ku80, respectively) and targets DNA-PKcs to ends of double-stranded DNA. DNA-PKcs is related by amino acid sequence to the phosphatidyl inositol kinase (PI-3 kinase) protein
licanming 请问有无人用过assaydesigns公司的PKC Kinase Activity Assay Kit（cat NO:EKS-420A）?可否说一下应用的经验？注意事项等？还有，该试剂盒的说明书有几处地方不是很懂，请大家帮忙看一下： 1、To avoid cross contamination, change disposable pipette tips between the addition of each standard
Measurement of Second Messengers in Signal Transduction: cAMP and Inositol Phosphates
. Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature 312:315‐321. Berridge, M.J., Dawson, R.M.C., Downes, C.P., Heslop, J.P., and Irvine, R.F
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