- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-27146h
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Huntingtin-interacting protein K,HYPK
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Huntingtin- interacting protein K, HYPK ELISA KIT
Product Name:Human Huntingtin- interacting protein K, HYPK ELISA KIT
Packing:96T
Catalog No.:ELI-27146h
Gene Name:HYPK
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Huntingtin-interacting protein K(HYPK
Alternative Name:HYPK,Huntingtin-interacting protein K(HYPK
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Huntingtin- interacting protein K, HYPK ELISA KIT allows for the in vitro quantitative determination of Huntingtin-interacting protein K(HYPK concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Huntingtin- interacting protein K, HYPK ELISA KIT has been pre-coated with an Huntingtin-interacting protein K(HYPK antibody specific to Huntingtin-interacting protein K(HYPK .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Huntingtin-interacting protein K(HYPK and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Huntingtin-interacting protein K(HYPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Huntingtin-interacting protein K(HYPK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Phage‐Based Expression Cloning to Identify Interacting Proteins
expression cloning is a simple, rapid, and powerful technique to identify interacting proteins. A protein of interest is expressed as a recombinant fusion protein and labeled with 32 P at an engineered recognition site to facilitate detection. b?gal proteins
Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins
pentagon, bait protein; open rectangle, noninteracting library protein; shaded box, activator protein (acid blob in Fig. ); open chevron, interacting library protein. View Image
technical steps to recover uPA and PAI-1 protein from archived breast cancer tissues and to quantitatively determine uPA and PAI-1 protein content in tumor tissue extracts by enzyme-linked immunosorbent assay (ELISA) are described in detail. The technical
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