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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
C-C motif chemokine 3-like 1,CCL3L1
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human C- C motif chemokine 3- like 1, CCL3L1 ELISA KIT
Product Name:Human C- C motif chemokine 3- like 1, CCL3L1 ELISA KIT
Packing:96T
Catalog No.:ELI-25675h
Gene Name:CCL3L1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:C-C motif chemokine 3-like 1(CCL3L1
Alternative Name:CCL3L1,C-C motif chemokine 3-like 1(CCL3L1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human C- C motif chemokine 3- like 1, CCL3L1 ELISA KIT allows for the in vitro quantitative determination of C-C motif chemokine 3-like 1(CCL3L1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human C- C motif chemokine 3- like 1, CCL3L1 ELISA KIT has been pre-coated with an C-C motif chemokine 3-like 1(CCL3L1 antibody specific to C-C motif chemokine 3-like 1(CCL3L1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for C-C motif chemokine 3-like 1(CCL3L1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C-C motif chemokine 3-like 1(CCL3L1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of C-C motif chemokine 3-like 1(CCL3L1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
in many signaling pathways typically associated with human malignancies. Phospho?motif antibodies are engineered to recognize proteins that contain a phosphorylated residue in the context of a specific motif. They are raised against a library of phospho?peptides
-associated kinase; ITAM, immunoreceptor tyrosine-based activation motif; JAK, Janus kinase; NFκB, nuclear factor κB; PKB, protein kinase B; PLC, phospholipase C; SIGIRR, single Ig IL-1-related receptor; sIL-1RAcP, soluble IL-1 receptor accessory
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
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