- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-25350h
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Serine/threonine-protein kinase B-raf,BRAF
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Serine/threonine- protein kinase B- raf, BRAF ELISA KIT
Product Name:Human Serine/threonine- protein kinase B- raf, BRAF ELISA KIT
Packing:96T
Catalog No.:ELI-25350h
Gene Name:BRAF
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Serine(threonine-protein kinase B-raf(BRAF
Alternative Name:BRAF,Serine(threonine-protein kinase B-raf(BRAF
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Serine/threonine- protein kinase B- raf, BRAF ELISA KIT allows for the in vitro quantitative determination of Serine(threonine-protein kinase B-raf(BRAF concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Serine/threonine- protein kinase B- raf, BRAF ELISA KIT has been pre-coated with an Serine(threonine-protein kinase B-raf(BRAF antibody specific to Serine(threonine-protein kinase B-raf(BRAF .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Serine(threonine-protein kinase B-raf(BRAF and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Serine(threonine-protein kinase B-raf(BRAF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Serine(threonine-protein kinase B-raf(BRAF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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