- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产(香港)
- ELI-23991h
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Acid ceramidase,ASAH1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Acid ceramidase, ASAH1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ASAH1,ACDase; ASAH; PHP; PHP32; SMAPME; acid CDase; acid ceramidase; acylsphingosine deacylase; N-acylsphingosine amidohydrolase (acid ceramidase) 1
Search name
Human ASAH1 ELISA KIT ,Human ACDase ELISA KIT ,Human ASAH ELISA KIT ,Human PHP ELISA KIT ,Human PHP32 ELISA KIT ,Human SMAPME ELISA KIT ,Human acid CDase ELISA KIT ,Human acid ceramidase ELISA KIT ,Human acylsphingosine deacylase ELISA KIT ,Human N-acylsphingosine amidohydrolase 1 ELISA KIT ,Human N-acylsphingosine acid ceramidase 1 ELISA KIT
Intended use
Human ASAH1 ELISA KIT allows for the in vitro quantitative determination of Human Acid ceramidase,ASAH1 beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Human ASAH1 ELISA KIT has been pre-coated with an antibody specific to Human Acid ceramidase,ASAH1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human Acid ceramidase,ASAH1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Acid ceramidase,ASAH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Acid ceramidase,ASAH1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human ASAH1 ELISA KIT Range 0.31-20ng/ml
ASAH1 ELISA KIT by email:风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Human Xanthine Oxidoreductase Determination by a Competitive ELISA
Xanthine oxidoreductase (XO) catalyses the oxidation of hypoxanthine to xanthine and of the latter to uric acid. The enzyme is present in traces in most of human tissues, including plasma, being more abundant in milk, liver, and intestine (1).
调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
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