- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-21561h
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
p53 apoptosis effector related to PMP-22,PERP
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human p53 apoptosis effector related to PMP- 22, PERP ELISA KIT
Product Name:Human p53 apoptosis effector related to PMP- 22, PERP ELISA KIT
Packing:96T
Catalog No.:ELI-21561h
Gene Name:PERP
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:p53 apoptosis effector related to PMP-22(PERP
Alternative Name:PERP,p53 apoptosis effector related to PMP-22(PERP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human p53 apoptosis effector related to PMP- 22, PERP ELISA KIT allows for the in vitro quantitative determination of p53 apoptosis effector related to PMP-22(PERP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human p53 apoptosis effector related to PMP- 22, PERP ELISA KIT has been pre-coated with an p53 apoptosis effector related to PMP-22(PERP antibody specific to p53 apoptosis effector related to PMP-22(PERP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for p53 apoptosis effector related to PMP-22(PERP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain p53 apoptosis effector related to PMP-22(PERP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of p53 apoptosis effector related to PMP-22(PERP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Guide to Cell Proliferation and Apoptosis Methods
Kit 11 Cell Death Detection ELISAPLUS 13 1.2.1.2 Assays that measure apoptosis-induced proteases (caspase) 16 Caspase
复合物 (Apoptosome)，后者聚集procaspase-9 5、 procaspase-9经自身裂解而活化。 6、Caspase-9裂解caspase-3和其它caspases，激活凋亡的效应器期 effector phase ）,从而使细胞结构被破坏。 五、IAPs, 抑制caspase 活性的蛋白家族 A. IAPs are inhibitors of apoptosis protein. There are 7 identified IAPs in human. B
细胞。 LXMSUNLIGHT： 1、流式细胞术检测细胞凋亡率 2、AO/EB荧光染色 （Wang CY, James E, Jim L. Spontaneous apoptosis in human colon tumor cell lines and the relation of WT P53 to apoptosis. Chinese Medical Journal, 1996;109:537-541） 方法略有改进：将处于对数生长期的2株细胞制成细胞悬液，调成1×109/L细胞密度加入细胞培养瓶，待细胞
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