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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
NADH-cytochrome b5 reductase 2,CYB5R2
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human NADH- cytochrome b5 reductase 2, CYB5R2 ELISA KIT
Product Name:Human NADH- cytochrome b5 reductase 2, CYB5R2 ELISA KIT
Packing:96T
Catalog No.:ELI-20060h
Gene Name:CYB5R2
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:NADH-cytochrome b5 reductase 2(CYB5R2
Alternative Name:CYB5R2,NADH-cytochrome b5 reductase 2(CYB5R2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human NADH- cytochrome b5 reductase 2, CYB5R2 ELISA KIT allows for the in vitro quantitative determination of NADH-cytochrome b5 reductase 2(CYB5R2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human NADH- cytochrome b5 reductase 2, CYB5R2 ELISA KIT has been pre-coated with an NADH-cytochrome b5 reductase 2(CYB5R2 antibody specific to NADH-cytochrome b5 reductase 2(CYB5R2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NADH-cytochrome b5 reductase 2(CYB5R2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NADH-cytochrome b5 reductase 2(CYB5R2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of NADH-cytochrome b5 reductase 2(CYB5R2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
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Intracellular Immunofluorescent Staining for Flow Cytometry
for detection of IL-6-producing cells by human LPS-activated monocytes is 6 hours, whereas IL-10 detection needs at least 24 hours stimulation. In contrast to detection of secreted cytokines by ELISA, for detection of intracellular cytokines
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