Human NADH-cytochrome b5 reduc

Human NADH- cytochrome b5 reductase 2, CYB5R2 ELISA KIT

Product Name:Human NADH- cytochrome b5 reductase 2, CYB5R2 ELISA KIT
Packing:96T

Catalog No.:ELI-20060h

Gene Name:CYB5R2

Detect Range:78-5000 pg/mL

Sensitivity:32pg/mL

Target Protein Name:NADH-cytochrome b5 reductase 2(CYB5R2

Alternative Name:CYB5R2,NADH-cytochrome b5 reductase 2(CYB5R2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human NADH- cytochrome b5 reductase 2, CYB5R2 ELISA KIT allows for the in vitro quantitative determination of NADH-cytochrome b5 reductase 2(CYB5R2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human NADH- cytochrome b5 reductase 2, CYB5R2 ELISA KIT has been pre-coated with an NADH-cytochrome b5 reductase 2(CYB5R2 antibody specific to NADH-cytochrome b5 reductase 2(CYB5R2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for NADH-cytochrome b5 reductase 2(CYB5R2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NADH-cytochrome b5 reductase 2(CYB5R2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of NADH-cytochrome b5 reductase 2(CYB5R2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!