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- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Golgin subfamily A member 7B,GOLGA7B
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Golgin subfamily A member 7B, GOLGA7B ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
GOLGA7B,C10orf132; C10orf133; bA451M19.3; bA459F3.4; Golgin subfamily A member 7B; golgi autoantigen, golgin subfamily a, 7B; golgin A7 family, member B
Search name
Human GOLGA7B ELISA KIT ,Human C10orf132 ELISA KIT ,Human C10orf133 ELISA KIT ,Human bA451M19.3 ELISA KIT ,Human bA459F3.4 ELISA KIT ,Human Golgin subfamily A member 7B ELISA KIT ,Human golgi autoantigen golgin subfamily a 7B ELISA KIT ,Human golgin A7 family member B ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Golgin subfamily A member 7B, GOLGA7B concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Golgin subfamily A member 7B, GOLGA7B, During the reaction, Human Golgin subfamily A member 7B, GOLGA7B in the sample or standard competes with a fixed amount of biotin-labeled Human Golgin subfamily A member 7B, GOLGA7B for sites on a pre-coated Monoclonal antibody specific to Human Golgin subfamily A member 7B, GOLGA7B. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Golgin subfamily A member 7B, GOLGA7B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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