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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
ICOS ligand,ICOSLG
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human ICOS ligand, ICOSLG ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ICOS ligand,LICOS,B7-related protein 1(B7RP-1),B7 homolog 2(B7-H2),B7-like protein Gl50,ICOSLG,B7-H2; B7H2; B7RP-1; B7RP1; CD275; GL50; ICOS-L; ICOSL; LICOS; B7 homolog 2; B7 homologue 2; B7-like protein Gl50; B7-related protein 1; ICOS ligand; transmembrane protein B7-H2 ICOS ligand; inducible T-cell co-stimulator ligand
Search name
Human ICOS ligand ELISA KIT ,Human LICOS ELISA KIT ,Human B7-related protein 1ELISA KIT ,Human B7 homolog 2(B7-H2) ELISA KIT ,Human B7-like protein Gl50 ELISA KIT ,Human ICOSLG ELISA KIT ,Human B7-H2 ELISA KIT ,Human B7H2 ELISA KIT ,Human B7RP-1 ELISA KIT ,Human B7RP1 ELISA KIT ,Human CD275 ELISA KIT ,Human GL50 ELISA KIT ,Human ICOS-L ELISA KIT ,Human ICOSL ELISA KIT ,Human LICOS ELISA KIT ,Human B7 homolog 2 ELISA KIT ,Human B7 homologue 2 ELISA KIT ,Human B7-like protein Gl50 ELISA KIT ,Human B7-related protein 1 ELISA KIT ,Human ICOS ligand ELISA KIT ,Human transmembrane protein B7-H2 ICOS ligand ELISA KIT ,Human inducible T-cell co-stimulator ligand ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human ICOS ligand concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to ICOS ligand. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ICOS ligand and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ICOS ligand, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ICOS ligand in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验28 Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use
-11, stem cell factor (SCF; also known as kit- ligand), and granulocyte-macrophage colony stimulating factor (GM-CSF) are also capable of promoting megakaryocytopoiesis and will synergize with TPO to increase megakaryocyte proliferation (reviewed in ref. 7 ).
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
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