Human ICOS ligand,ICOSLG ELISA

Human ICOS ligand, ICOSLG ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

ICOS ligand,LICOS,B7-related protein 1(B7RP-1),B7 homolog 2(B7-H2),B7-like protein Gl50,ICOSLG,B7-H2; B7H2; B7RP-1; B7RP1; CD275; GL50; ICOS-L; ICOSL; LICOS; B7 homolog 2; B7 homologue 2; B7-like protein Gl50; B7-related protein 1; ICOS ligand; transmembrane protein B7-H2 ICOS ligand; inducible T-cell co-stimulator ligand

Search name

Human ICOS ligand ELISA KIT ,Human LICOS ELISA KIT ,Human B7-related protein 1ELISA KIT ,Human B7 homolog 2(B7-H2) ELISA KIT ,Human B7-like protein Gl50 ELISA KIT ,Human ICOSLG ELISA KIT ,Human B7-H2 ELISA KIT ,Human B7H2 ELISA KIT ,Human B7RP-1 ELISA KIT ,Human B7RP1 ELISA KIT ,Human CD275 ELISA KIT ,Human GL50 ELISA KIT ,Human ICOS-L ELISA KIT ,Human ICOSL ELISA KIT ,Human LICOS ELISA KIT ,Human B7 homolog 2 ELISA KIT ,Human B7 homologue 2 ELISA KIT ,Human B7-like protein Gl50 ELISA KIT ,Human B7-related protein 1 ELISA KIT ,Human ICOS ligand ELISA KIT ,Human transmembrane protein B7-H2 ICOS ligand ELISA KIT ,Human inducible T-cell co-stimulator ligand ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Human ICOS ligand concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to ICOS ligand. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ICOS ligand and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ICOS ligand, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ICOS ligand in the samples is then determined by comparing the O.D. of the samples to the standard curve.