- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产(香港)
- ELI-20509h
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
ICOS ligand,ICOSLG
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human ICOS ligand, ICOSLG ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ICOS ligand,LICOS,B7-related protein 1(B7RP-1),B7 homolog 2(B7-H2),B7-like protein Gl50,ICOSLG,B7-H2; B7H2; B7RP-1; B7RP1; CD275; GL50; ICOS-L; ICOSL; LICOS; B7 homolog 2; B7 homologue 2; B7-like protein Gl50; B7-related protein 1; ICOS ligand; transmembrane protein B7-H2 ICOS ligand; inducible T-cell co-stimulator ligand
Search name
Human ICOS ligand ELISA KIT ,Human LICOS ELISA KIT ,Human B7-related protein 1ELISA KIT ,Human B7 homolog 2(B7-H2) ELISA KIT ,Human B7-like protein Gl50 ELISA KIT ,Human ICOSLG ELISA KIT ,Human B7-H2 ELISA KIT ,Human B7H2 ELISA KIT ,Human B7RP-1 ELISA KIT ,Human B7RP1 ELISA KIT ,Human CD275 ELISA KIT ,Human GL50 ELISA KIT ,Human ICOS-L ELISA KIT ,Human ICOSL ELISA KIT ,Human LICOS ELISA KIT ,Human B7 homolog 2 ELISA KIT ,Human B7 homologue 2 ELISA KIT ,Human B7-like protein Gl50 ELISA KIT ,Human B7-related protein 1 ELISA KIT ,Human ICOS ligand ELISA KIT ,Human transmembrane protein B7-H2 ICOS ligand ELISA KIT ,Human inducible T-cell co-stimulator ligand ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human ICOS ligand concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to ICOS ligand. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ICOS ligand and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ICOS ligand, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ICOS ligand in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
操作条件、人员一致 边缘效应 蒸发/酶标板叠放 孵育加封板膜或盖板；避免叠放 孵育温度不均 校准温育箱；避开通风口、窗边等环境波动区 洗板不彻底 确保洗板仪正常；不减少洗液量与次数 注意！！！ ELISA试剂盒11月活动即将来袭~敬请期待~ 部分热卖产品推荐 产品货号 产品名称 检测范围 灵敏度 97109ES Human IL18 ELISA Kit 125
28 Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use
-11, stem cell factor (SCF; also known as kit- ligand), and granulocyte-macrophage colony stimulating factor (GM-CSF) are also capable of promoting megakaryocytopoiesis and will synergize with TPO to increase megakaryocyte proliferation (reviewed in ref. 7 ).
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