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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Probable protein phosphatase 1N,PPM1N
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Probable protein phosphatase 1N, PPM1N ELISA KIT
Product Name:Human Probable protein phosphatase 1N, PPM1N ELISA KIT
Packing:96T
Catalog No.:ELI-19718h
Gene Name:PPM1N
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Probable protein phosphatase 1N(PPM1N
Alternative Name:PPM1N,Probable protein phosphatase 1N(PPM1N
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Probable protein phosphatase 1N, PPM1N ELISA KIT allows for the in vitro quantitative determination of Probable protein phosphatase 1N(PPM1N concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Probable protein phosphatase 1N, PPM1N ELISA KIT has been pre-coated with an Probable protein phosphatase 1N(PPM1N antibody specific to Probable protein phosphatase 1N(PPM1N .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Probable protein phosphatase 1N(PPM1N and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Probable protein phosphatase 1N(PPM1N, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Probable protein phosphatase 1N(PPM1N in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dehydrogenases. Progr. Histochem. Cytochem . 4 :225. Berger, J., A.D. Howard, L. Gerber, B.R. Cullen, and S. Udenfriend. 1987. Expression of active, membrane-bound human placental alkaline phosphatase by transfected simian cells. Proc. Natl. Acad. Sci
4) 0.87 gSodium phosphate monobasic (Na2HPO4* H2O) 0.54 gSodium chloride 8.5 gHPLC water 1.00 LAdgust PH to 7.4 with 1N NaOH 1M HClSLGMA Immunp Chemical SIGMA DiagnosticsAnti-mouse IgG (Fab specific) Sigma 104 PhosphataseAlaline Phosphatase conjugate
Identifying Small‐Molecule Modulators of Protein‐Protein Interactions
. Horswill, A.R., Savinov, S.N., and Benkovic, S.J. 2004. A systematic method for identifying small‐molecule modulators of protein‐protein interactions. Proc. Natl. Acad. Sci. U.S.A 101:15591‐15596
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