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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Prostamide/prostaglandin F synthase,C1orf93
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Prostamide/prostaglandin F synthase, C1orf93 ELISA KIT
Product Name:Human Prostamide/prostaglandin F synthase, C1orf93 ELISA KIT
Packing:96T
Catalog No.:ELI-15248h
Gene Name:C1orf93
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Prostamide(prostaglandin F synthase(C1orf93
Alternative Name:C1orf93,Prostamide(prostaglandin F synthase(C1orf93
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Prostamide/prostaglandin F synthase, C1orf93 ELISA KIT allows for the in vitro quantitative determination of Prostamide(prostaglandin F synthase(C1orf93 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Prostamide/prostaglandin F synthase, C1orf93 ELISA KIT has been pre-coated with an Prostamide(prostaglandin F synthase(C1orf93 antibody specific to Prostamide(prostaglandin F synthase(C1orf93 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Prostamide(prostaglandin F synthase(C1orf93 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Prostamide(prostaglandin F synthase(C1orf93, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Prostamide(prostaglandin F synthase(C1orf93 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂,增加了操作步骤,在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法:1, TORCH及传染病试剂盒(间接法),见2.2.32, TORCH-IgM捕获法特色:包被抗体,标记抗原原理:3, 细胞因子试剂盒采用的方法路线(ABC-ELISA)原理产品特色:采用ABC法,灵敏度更高,特异性更强。生物素抗体和酶联物是浓缩的,使用前需用相应的缓冲液稀释。酶联物可以通用
. J Biol Chem. 274 , 10681–10684. 10. Ding VW, Chen RH, McCormick F. (2000) Differential regulation of glycogen synthase kinase 3beta by insulin and Wnt signaling. J Biol Chem. 275 , 32475–32481. 11. Hoeflich KP, Luo J, Rubie EA, Tsao
Scanning the human genome with combinatorial transcription factor libraries Published online: 18 February 2003, doi:10.1038/nbt794 March 2003 Volume 21 Number 3 pp 269 - 274 Pilar Blancafort, Laurent Magnenat & Carlos F
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