- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-15186h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
60 kDa SS-A/Ro ribonucleoprotein,TROVE2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human 60 kDa SS- A/Ro ribonucleoprotein, TROVE2 ELISA KIT
Product Name:Human 60 kDa SS- A/Ro ribonucleoprotein, TROVE2 ELISA KIT
Packing:96T
Catalog No.:ELI-15186h
Gene Name:TROVE2
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:60 kDa SS-A(Ro ribonucleoprotein(TROVE2
Alternative Name:TROVE2,60 kDa SS-A(Ro ribonucleoprotein(TROVE2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human 60 kDa SS- A/Ro ribonucleoprotein, TROVE2 ELISA KIT allows for the in vitro quantitative determination of 60 kDa SS-A(Ro ribonucleoprotein(TROVE2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human 60 kDa SS- A/Ro ribonucleoprotein, TROVE2 ELISA KIT has been pre-coated with an 60 kDa SS-A(Ro ribonucleoprotein(TROVE2 antibody specific to 60 kDa SS-A(Ro ribonucleoprotein(TROVE2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for 60 kDa SS-A(Ro ribonucleoprotein(TROVE2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 60 kDa SS-A(Ro ribonucleoprotein(TROVE2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of 60 kDa SS-A(Ro ribonucleoprotein(TROVE2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
Figure 17.15.4 Defining a useful dilution of human plasma for measuring 3‐nitrotyrosine. ELISA microarray results for the dilution of human plasma. Four samples were diluted with 0.1% BSA/PBS at 2‐, 5‐, 10
引物的延伸。②循环后期酶量渐感不足而产生平坡。到达平玻的循环次数，Klenow酶为20个（均用1μg基因组DNA开始）而Taq酶为30个。③延伸片段长度Taq酶为10kb以内，而Klenow酶为400bp以内。 Taq酶由水栖高温菌（Thermus aquatics）YT1蓖株中分离而得。此菌于1969年由Brock分离自美国黄石公园温泉，作为栖热杆菌的标准菌株，其生长温度为70～75℃。最初从中分离到分子量60～68KDa，比活性为2000～8000U/mg的DNA聚合酶。后来Cetus公司
剂如Triton X-100、脱氧胆酸盐和低浓度的变性剂如尿素充分洗涤去除杂质,这一步很重要,因为大肠杆菌外膜蛋白Omp T(37 KDa)在4-8mol/L尿素中具有蛋白水解酶活性,在包涵体的溶解和复性过程中可导致重组蛋白质的降解。 对于尿素和盐酸胍的选择： 尿素和盐酸胍属中强度变性剂，易经透析和超滤除去。它们对包涵体氢键有较强的可逆性变性作用，所需浓度尿素8-10M，盐酸胍6-8M。尿素溶解包涵体较盐酸胍慢而弱，溶解度为70-90%，尿素在作用时间较长或温度较高时会裂解形成氰酸盐，对重组蛋白质的氨
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