- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-15174h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Prohibitin-2,PHB2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Prohibitin- 2, PHB2 ELISA KIT
Product Name:Human Prohibitin- 2, PHB2 ELISA KIT
Packing:96T
Catalog No.:ELI-15174h
Gene Name:PHB2
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Prohibitin-2(PHB2
Alternative Name:PHB2,Prohibitin-2(PHB2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Prohibitin- 2, PHB2 ELISA KIT allows for the in vitro quantitative determination of Prohibitin-2(PHB2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Prohibitin- 2, PHB2 ELISA KIT has been pre-coated with an Prohibitin-2(PHB2 antibody specific to Prohibitin-2(PHB2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Prohibitin-2(PHB2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Prohibitin-2(PHB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Prohibitin-2(PHB2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验外膜上，而后与LC3II结合介导线粒体自噬。来自武汉大学生命科学学院宋质银教授研究组发表了题为“PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis （PHB2通过PRAL-PGAM5-PINK1轴线促进PINK1-Parkin介导的线粒体自噬）”的最新研究成果，揭示了PHB2调节线粒体自噬的新的分子机制，而且发现了以PHB2为靶点通过阻止线粒体自噬来抑制肿瘤细胞的生存可作
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
的原理用于分子诊断方面：TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的，相关文章见：1，Rapid screen for truncating ATM mutations by PTT-ELISA . 2，An ELISA-based high throughput protein truncation test
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