Human Protein Wnt- 10b, WNT10B

Human Protein Wnt-10b, Wnt10b ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Protein Wnt-10b,Protein Wnt-12,; WNT10B,SHFM6; WNT-12; WNT-10B protein; protein Wnt-10b; wingless-type MMTV integration site family member 10B,Wnt12; wingless related MMTV integration site 10b

Search name

Human Protein Wnt-10b ELISA KIT ,Human Protein Wnt-12 ELISA KIT ,Human WNT10B ELISA KIT ,Human SHFM6 ELISA KIT ,Human WNT-12 ELISA KIT ,Human WNT-10B protein ELISA KIT ,Human protein Wnt-10b ELISA KIT ,Human wingless-type MMTV integration site family member 10B ELISA KIT ,Human Wnt12 ELISA KIT ,Human wingless related MMTV integration site 10b ELISA KIT

Intended use

Human Wnt10b ELISA Kit allows for the in vitro quantitative determination of human Protein Wnt-10b concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Human Wnt10b ELISA Kit  has been pre-coated with an antibody specific to Protein Wnt-10b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Protein Wnt-10b and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein Wnt-10b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Protein Wnt-10b in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Human Wnt10b ELISA Kit  PDF Manual download