- ￥2890
- LSM Bio
- 参照说明书
- 0.31-20ng/ml
- 国产
- ELI-12214h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
参照说明书
- 检测方法：
夹心法/竞争法
- 应用：
0.1ng/ml
- 适应物种：
人
- 标记物：
Neuronal acetylcholine receptor subunit alpha- 7, CHRNA7 E
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.31-20ng/ml
- 规格：
96T
Human Neuronal acetylcholine receptor subunit alpha- 7, CHRNA7 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CHRNA7,CHRNA7-2; NACHRA7; a7 nicotinic acetylcholine receptor; alpha 7 neuronal nicotinic acetylcholine receptor; alpha-7 nicotinic cholinergic receptor subunit; cholinergic receptor, nicotinic alpha 7; cholinergic receptor, nicotinic, alpha 7 (neuronal); cholinergic receptor, nicotinic, alpha polypeptide 7; neuronal acetylcholine receptor protein, alpha-7 chain; cholinergic receptor nicotinic alpha 7 subunit
Search name
Human CHRNA7 ELISA KIT ,Human CHRNA7-2 ELISA KIT ,Human NACHRA7 ELISA KIT ,Human a7 nicotinic acetylcholine receptor ELISA KIT ,Human alpha 7 neuronal nicotinic acetylcholine receptor ELISA KIT ,Human alpha-7 nicotinic cholinergic receptor subunit ELISA KIT ,Human cholinergic receptor nicotinic alpha 7 ELISA KIT ,Human cholinergic receptor nicotinic alpha 7 neuronal ELISA KIT ,Human cholinergic receptor nicotinic alpha polypeptide 7 ELISA KIT ,Human neuronal acetylcholine receptor protein alpha-7 chain ELISA KIT ,Human cholinergic receptor nicotinic alpha 7 subunit ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Neuronal acetylcholine receptor subunit alpha- 7, CHRNA7 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Neuronal acetylcholine receptor subunit alpha-7,CHRNA7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human Neuronal acetylcholine receptor subunit alpha-7,CHRNA7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Neuronal acetylcholine receptor subunit alpha-7,CHRNA7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Neuronal acetylcholine receptor subunit alpha-7,CHRNA7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Strategies to Optimize Protein Expression in E. coli
Tanaka, T., Kubota, M., Samizo, K., Nakajima, Y., Hoshino, M., Kohno, T., and Wakamatsu, E. 1999. One‐step affinity purification of the G protein beta gamma subunits from bovine brain using a histidine‐tagged G protein alpha subunit. Protein Expr
Introduction of human 14-3-3 proteins
association of 14-3-3 proteins with Cbl14-3-3 binding sequence in cytoplasmic domain of adhesion receptor,platelet glycoprotein Ib alpha--------------------------------------------------------------------------------14-3-3 brain protein homologs in plantsThe
Recombinant Cell Lines Stably Expressing Functional Ion Channels
acetylcholine receptor alpha7 (nAChRα7) in a host cell, we show a way to construct and use such a stable cell-based expression system for electrophysiological assays.
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