- ￥2890
- LSM Bio
- 参照说明书
- 0.31-20ng/ml
- 国产
- ELI-11400h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
参照说明书
- 检测方法：
夹心法/竞争法
- 应用：
0.1ng/ml
- 适应物种：
人
- 标记物：
Cyclin- F, CCNF
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.31-20ng/ml
- 规格：
96T
Human Cyclin- F, CCNF ELISA KIT
Product Name:Human Cyclin- F, CCNF ELISA KIT
Packing:96T
Catalog No.:ELI-11400h
Gene Name:CCNF
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Cyclin-F(CCNF
Alternative Name:CCNF,Cyclin-F(CCNF
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cyclin- F, CCNF ELISA KIT allows for the in vitro quantitative determination of Cyclin-F(CCNF concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cyclin- F, CCNF ELISA KIT has been pre-coated with an Cyclin-F(CCNF antibody specific to Cyclin-F(CCNF .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cyclin-F(CCNF and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cyclin-F(CCNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cyclin-F(CCNF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr（图）
to detect cyclin D1 expression in several tumor cell lines, activated human lymphocytes and chick chondrocytes. Below, HL-60 cells labeled for cyclin D1 or cyclin D2/D3 with 80% EtOH treatment at –20℃. References Gong, J., Bhatia, U
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometry
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