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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
参照说明书
- 检测方法:
夹心法/竞争法
- 应用:
0.1ng/ml
- 适应物种:
人
- 标记物:
Cytochrome b- 245 light chain, CYBA
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.31-20ng/ml
- 规格:
96T
Human Cytochrome b- 245 light chain, CYBA ELISA KIT
Product Name:Human Cytochrome b- 245 light chain, CYBA ELISA KIT
Packing:96T
Catalog No.:ELI-10312h
Gene Name:CYBA
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Cytochrome b-245 light chain(CYBA
Alternative Name:CYBA,Cytochrome b-245 light chain(CYBA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cytochrome b- 245 light chain, CYBA ELISA KIT allows for the in vitro quantitative determination of Cytochrome b-245 light chain(CYBA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cytochrome b- 245 light chain, CYBA ELISA KIT has been pre-coated with an Cytochrome b-245 light chain(CYBA antibody specific to Cytochrome b-245 light chain(CYBA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cytochrome b-245 light chain(CYBA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome b-245 light chain(CYBA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cytochrome b-245 light chain(CYBA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验【分享】Human press METHODS IN MOLECULAR BIOLOGY
and Protocols. Edited by Pascol Bailon, 2000 148.DNA-protein interactions, Principle and protocols, 2nd, Edited by Tom Moss, 2000 149.The ELISA guide book, Edited by John Crowther, 2000 150.Complement, Methods and protocols. Edited by B. Paul Morgan. 2000
Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases
, all of which are subunits of the respiratory chain complexes. Of the 13 structural genes, 7 encode subunits of complex I (NADH-CoQ oxidoreductase), 1 encodes the cytochrome-b subunit of complex III (CoQ-cytochrome-c oxidoreductase), 3 encode subunits of complex IV
线粒体荧光探针大全:TMRM,Mitotracker,JC-1
equilibration of ions leads in turn to the decoupling of the respiratory chain and then the release of cytochrome c into the cytosol.188,189 The SelectFX Alexa Fluor 488 Cytochrome c Apoptosis Detection Kit (S35115) provides all the reagents required to detect
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