- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 进口
- ELI-40760h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Tight junction protein ZO-1,TJP1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Tight junction protein ZO- 1, TJP1 ELISA KIT
Product Name:Human Tight junction protein ZO- 1, TJP1 ELISA KIT
Packing:96T
Catalog No.:ELI-40760h
Gene Name:TJP1
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Tight junction protein ZO-1(TJP1
Alternative Name:TJP1,Tight junction protein ZO-1(TJP1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Tight junction protein ZO- 1, TJP1 ELISA KIT allows for the in vitro quantitative determination of Tight junction protein ZO-1(TJP1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Tight junction protein ZO- 1, TJP1 ELISA KIT has been pre-coated with an Tight junction protein ZO-1(TJP1 antibody specific to Tight junction protein ZO-1(TJP1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Tight junction protein ZO-1(TJP1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tight junction protein ZO-1(TJP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Tight junction protein ZO-1(TJP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验. In addition, methods for assessing mRNA expression and protein content of the main TJ proteins (occludin, zonula occludens-1 [ZO-1]) are detailed. Finally, we provide the methods required for confocal immunofluorescence detection of the TJ proteins
Dynamics of Claudins Expression in Colitis and Colitis-Associated Cancer in Rat
Claudins comprise a multigene family of 24 species and have been shown to constitute the backbone of tight junction strands in simple epithelial cells and to be directly involved in their barrier functions. Apical-most tight junction protein
Biochemical Assays for Studying Indirect Interactions Between CFTR and the Cytoskeleton
-A, and the tight junction protein ZO-1 (reviewed in refs. 1 and 2 ). PDZ domains typically mediate interactions with the COOH termini of proteins terminating in consensus PDZ-binding sequences (E-S/T-X-V/I), although other types of interaction also occur
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