- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-07760h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Poly [ADP- ribose] polymerase 2, PARP2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Poly [ADP- ribose] polymerase 2, PARP2 ELISA KIT
Product Name:Human Poly [ADP- ribose] polymerase 2, PARP2 ELISA KIT
Packing:96T
Catalog No.:ELI-07760h
Gene Name:PARP2
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Poly [ADP-ribose] polymerase 2(PARP2
Alternative Name:PARP2,Poly [ADP-ribose] polymerase 2(PARP2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Poly [ADP- ribose] polymerase 2, PARP2 ELISA KIT allows for the in vitro quantitative determination of Poly [ADP-ribose] polymerase 2(PARP2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Poly [ADP- ribose] polymerase 2, PARP2 ELISA KIT has been pre-coated with an Poly [ADP-ribose] polymerase 2(PARP2 antibody specific to Poly [ADP-ribose] polymerase 2(PARP2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Poly [ADP-ribose] polymerase 2(PARP2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Poly [ADP-ribose] polymerase 2(PARP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Poly [ADP-ribose] polymerase 2(PARP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验A general method to express and purify full-length human poly(ADP-ribose) polymerase-1 (PARP-1), individual PARP-1 domains, and groups of PARP-1 domains from Escherichia coli cells is described. The procedure allows for robust production
Evaluation of Tankyrase Inhibition in Whole Cells
The telomeric poly(ADP-ribose) polymerase (PARP), tankyrase 1, modulates the impact of telomerase inhibition on human cancer cells. Thus, overexpression of tankyrase 1 in telomerase-positive cancer cells confers resistance to telomerase
PARP1 Genomics: Chromatin Immunoprecipitation Approach Using Anti-PARP1 Antibody (ChIP and ChIP-seq)
Poly(ADP-ribose) polymerase1 (PARP1) is a global regulator of different cellular mechanisms, ranging from DNA damage repair to control of gene expression. Since PARP1 protein and pADPr have been shown to persist in chromatin through cell
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