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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Eosinophil peroxidase, EPX
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Eosinophil peroxidase,EPX ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY!
Alternative name
Human EPX ELISA KIT ,Human osinophil peroxidase ELISA KIT ,Human EPX-PEN ELISA KIT ,Human EPO ELISA KIT ,Human EPP ELISA KIT ,Human EPXD ELISA KIT ,Human Eosinophil peroxidase ELISA KIT,Human EPER ELISA KIT
Intended use
Human EPX ELISA KIT allows for the in vitro quantitative determination of human eosinophil peroxidase,EPX concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Human EPX ELISA KIT has been pre-coated with an antibody specific to EPX. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EPX and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain EPX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EPX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 78.0-5000 pg/ml
PDF Manual download
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