- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-06892h
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Dual specificity protein phosphatase 2, DUSP2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Dual specificity protein phosphatase 2, DUSP2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
DUSP2,PAC-1; PAC1; dual specificity protein phosphatase 2; dual specificity protein phosphatase PAC-1; dual-specificity phosphatase 2; serine/threonine specific protein phosphatase; dual specificity phosphatase 2
Search name
Human DUSP2 ELISA KIT ,Human PAC-1 ELISA KIT ,Human PAC1 ELISA KIT ,Human dual specificity protein phosphatase 2 ELISA KIT ,Human dual specificity protein phosphatase PAC-1 ELISA KIT ,Human dual-specificity phosphatase 2 ELISA KIT ,Human serine/threonine specific protein phosphatase ELISA KIT ,Human dual specificity phosphatase 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Dual specificity protein phosphatase 2, DUSP2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Dual specificity protein phosphatase 2, DUSP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Human Dual specificity protein phosphatase 2, DUSP2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Dual specificity protein phosphatase 2, DUSP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Dual specificity protein phosphatase 2, DUSP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Functional Studies of Dual-Specificity Phosphatases
Dual-speclflcity phosphatases, a subfamily enzymes of protein tyrosine phosphatases, play important roles in signal transduction and cell-cycle regulation during cell growth and differentiation (1 ). Unlike classic tyrosme phosphatases
Discovery of Protein Kinase Phosphatase Inhibitors
with chemical biology and high-content multiparametric analyses and was developed as a screening approach to discover protein phosphatase inhibitors. Using a dual specificity mitogen-activated protein kinase phosphatase as an example, a detailed protocol
Dual- and Triple-Color Fluorospot
实验原理 ELISPOT has, due to its high sensitivity and simplicity, proven valuable for assessing antigen-specific T-cell responses, both with regard to specificity and magnitude. One limitation of the regular ELISPOT method
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