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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Creatine kinase B- type, CKB
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Creatine kinase B- type, CKB ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Creatine kinase B-type,B-CK,Creatine kinase B chain, creatine kinase, brain; creatine kinase-B; creatine kinase B-type; creatine kinase B
chain; B-CK; CKBB,CKB
Search name
Human Creatine kinase B-type ELISA KIT ,Human B-CK ELISA KIT ,Human Creatine kinase B chain ELISA KIT ,Human creatine kinase ELISA KIT ,Human brain ELISA KIT ,Human creatine kinase-B ELISA KIT ,Human creatine kinase B-type ELISA KIT ,Human creatine kinase B chain ELISA KIT ,Human B-CK ELISA KIT ,Human CKBB ELISA KIT ,Human CKB ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human creatine kinase b-type,Creatine kinase B-type
concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine kinase B-type. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Creatine kinase
B-type and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate
solution is added to each well. Only those wells that contain Creatine kinase B-type, biotin-conjugated antibody and enzyme-conjugated
Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color
change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Creatine kinase B-type in the
samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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