- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-05555h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Pyridoxal phosphate phosphatase, PDXP
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Pyridoxal phosphate phosphatase, PDXP ELISA KIT
Product Name:Human Pyridoxal phosphate phosphatase, PDXP ELISA KIT
Packing:96T
Catalog No.:ELI-05555h
Gene Name:PDXP
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Pyridoxal phosphate phosphatase(PDXP
Alternative Name:PDXP,Pyridoxal phosphate phosphatase(PDXP
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Pyridoxal phosphate phosphatase, PDXP ELISA KIT allows for the in vitro quantitative determination of Pyridoxal phosphate phosphatase(PDXP concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Pyridoxal phosphate phosphatase, PDXP ELISA KIT has been pre-coated with an Pyridoxal phosphate phosphatase(PDXP antibody specific to Pyridoxal phosphate phosphatase(PDXP .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Pyridoxal phosphate phosphatase(PDXP and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pyridoxal phosphate phosphatase(PDXP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Pyridoxal phosphate phosphatase(PDXP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Human Placental Alkaline Phosphatase Stain
The following protocol is for detection of human placental alkaline phosphatase (AP) in cultured cells. Human placental AP is heat stable, unlike other AP proteins typically expressed by cells. A few cell types (HeLa cells, for example
to a goat antimouse IgG, which catalyzes the substrate p-nitrophenyl phosphate, to form a colored product. Excess reagents are removed by washing, and enzyme activity can be measured with an ELISA plate reader at 415nm. The amount of colored product
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dehydrogenases. Progr. Histochem. Cytochem . 4 :225. Berger, J., A.D. Howard, L. Gerber, B.R. Cullen, and S. Udenfriend. 1987. Expression of active, membrane-bound human placental alkaline phosphatase by transfected simian cells. Proc. Natl. Acad. Sci
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