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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Proheparin- binding EGF- like growth factor, HBEGF ELISA K
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Proheparin-binding EGF-like growth factor, HBEGF ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Proheparin-binding EGF-like growth factor, HBEGF,HB-EGF,DTR; DTS; DTSF; HEGFL; diphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor); heparin-binding epidermal growth factor; proheparin-binding EGF-like growth factor; heparin-binding EGF-like growth factor
Search name
Human Proheparin-binding EGF-like growth factor ELISA KIT ,Human HBEGF ELISA KIT ,Human HB-EGF ELISA KIT ,Human DTR ELISA KIT ,Human DTS ELISA KIT ,Human DTSF ELISA KIT ,Human HEGFL ELISA KIT ,Human diphtheria toxin receptor (heparin-binding EGF-like growth factor) ELISA KIT ,Human diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) ELISA KIT ,Human heparin-binding epidermal growth factor ELISA KIT ,Human proheparin-binding EGF-like growth factor ELISA KIT ,Human heparin-binding EGF-like growth factor ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human proheparin-binding egf-like growth factor,HBEGF concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HBEGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HBEGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HBEGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HBEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验webowner1985 如题。要是用一种药物处理细胞EGF分泌增多,请问参与促进EGF分泌的通路是什么呢?谢谢 zongjieli82 Cheung,W.Y., Zhai,R., Kulke,M., Heist,R., Asomaning,K., Ma,C., Wang,Z., Su,L., Christiani,D. and Liu,G. Epidermal Growth Factor A61G Gene
Regulator of Epidermal Growth Factor Signaling: Sprouty
Sprouty was first discovered through its downregulatory effect on fibroblast growth factor (FGF) receptor pathway during tracheal development. Sprouty expression is also induced by the epidermal growth factor receptor (EGFR) cascade
factor (EGF) receptor also stimulates second-messenger systems that involve the heterotrimeric G proteins. For instance, in the heart EGF increases contractility and heart rate by elevating cellular cyclic adenosine monophosphate (cAMP) levels
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