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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Laminin subunit alpha 5, LAMA5
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Laminin subunit alpha 5, LAMA5 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Laminin subunit alpha-5,Laminin-15 subunit alpha,Laminin-10 subunit alpha,Laminin-11 subunit alpha, LAMA5,laminin alpha-5 chain; laminin subunit alpha-5; laminin-10 subunit alpha; laminin-11 subunit alpha; laminin-15 subunit alpha; laminin alpha 5
Search name
Human Laminin subunit alpha-5 ELISA KIT ,Human Laminin-15 subunit alpha ELISA KIT ,Human Laminin-10 subunit alpha ELISA KIT ,Human Laminin-11 subunit alpha ELISA KIT ,Human LAMA5 ELISA KIT ,Human laminin alpha-5 chain ELISA KIT ,Human laminin subunit alpha-5 ELISA KIT ,Human laminin-10 subunit alpha ELISA KIT ,Human laminin-11 subunit alpha ELISA KIT ,Human laminin-15 subunit alpha ELISA KIT ,Human laminin alpha 5 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human Laminin subunit alpha-5 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin subunit alpha-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Laminin subunit alpha-5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Laminin subunit alpha-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Laminin subunit alpha-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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