- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-04766h
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Carboxypeptidase M, CPM
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Carboxypeptidase M, CPM ELISA KIT
Product Name:Human Carboxypeptidase M, CPM ELISA KIT
Packing:96T
Catalog No.:ELI-04766h
Gene Name:CPM
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Carboxypeptidase M(CPM
Alternative Name:CPM,Carboxypeptidase M(CPM
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Carboxypeptidase M, CPM ELISA KIT allows for the in vitro quantitative determination of Carboxypeptidase M(CPM concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Carboxypeptidase M, CPM ELISA KIT has been pre-coated with an Carboxypeptidase M(CPM antibody specific to Carboxypeptidase M(CPM .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Carboxypeptidase M(CPM and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Carboxypeptidase M(CPM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Carboxypeptidase M(CPM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验80150ml of PBS , 0.01 M, PH 7.4Reference/More informationVoller A , Bartlett A, Bidwell DE, Clark MF, Adams An, 1976. The detection of viruses by enzyme-linked immunosorbent assay (ELISA). J Gen Virol 33, 165-167.Figure 1
pneumoniae , has necessitated a reliance by diagnostic laboratories on serological techniques that are beyond the scope of this text but are summarized in Table 1 . Table 1 An Evaluation of Serological Tests for M. Pneumoniae
PriCells: Isolation, culture, and purification of Human Pulmonary Microvascular Endothelial Cells
and erythrocytes were removed by filtration through a 100-m nylon mesh strainer (Becton Dickinson Plasticware). 5. The tissue fragments were digested with PriCells Isolation Kit for 30 minutes at 37° C after which the suspension was homogenized by pipetting
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