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细胞污染识别与处理全攻略:5 种常见类型+关键误区Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Alpha- crystallin A chain, CRYAA
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human CRYAA ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Human CRYAA ELISA Kit, Human CRYA1 ELISA Kit, Human CTRCT9 ELISA Kit, Human HSPB4 ELISA Kit, Human alpha-crystallin A chain ELISA Kit, Human crystallin alpha-1 ELISA Kit, Human heat shock protein beta-4 ELISA Kit,human alphaA-crystallin (CRYA1) ELISA Kit, Human crystallin alpha A ELISA Kit
Intended use
Human CRYAA ELISA kit is for the in vitro quantitative determination of Human CRYAA concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Human CRYAA ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CRYAA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CRYAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CRYAA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human CRYAA ELISA kit Range 0.15-10ng/ml
CRYAA Related Products
> CRYAA ELISA KITS
Bovine Alpha- crystallin A chain, CRYAA ELISA KIT
Canine Alpha- crystallin A chain, CRYAA ELISA KIT
Chicken Alpha- crystallin A chain, CRYAA ELISA KIT
Human Alpha- crystallin A chain, CRYAA ELISA KIT
Human Crystallin Alpha A,CRYaA ELISA Kit
Mouse Alpha- crystallin A chain, Cryaa ELISA KIT
Porcine Alpha- crystallin A chain, CRYAA ELISA KIT
Rabbit Alpha- crystallin A chain, CRYAA ELISA KIT
Rat Alpha- crystallin A chain, Cryaa ELISA KIT
> CRYAA Recombinant Protein
Human Alpha Crystallin A, CRYAA Recombinant Protein
Human CRYAA (Crystallin alpha A) , CRYAA Recombinant Protein
> CRYAA Antidodies
Anti CRYAA Antidody
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文献和实验Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
调至 1μg/μl。 4. ELISA 检测:按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数,结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理,全程低温操作,裂解液现配现用; 2. 蛋白定量时标准品与样本同步孵育,稀释采用倍比稀释减少误差; 3. ELISA 实验需每次重绘标准曲线,洗板充分、严格把控显色与终止时间。 免疫检测试剂盒: 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
Introduction of human 14-3-3 proteins
regulation.We report here the crystal structure of the human T-cell 14-3-3 isoform (tau)dimer at 2.6 A resolution.Each monomer (Mr 28K)is composed of an unusual arrangement of nine antiparallel alpha-helices organized as two structural domains.The dimer
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