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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Insulin Autoantibodies, IAA
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Insulin Autoantibodies, IAA ELISA KIT
Product Name:Human Insulin Autoantibodies, IAA ELISA KIT
Packing:96T
Catalog No.:ELI-04174h
Gene Name:IAA
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Insulin Autoantibodies(IAA
Alternative Name:IAA,Insulin Autoantibodies(IAA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Insulin Autoantibodies, IAA ELISA KIT allows for the in vitro quantitative determination of Insulin Autoantibodies(IAA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Insulin Autoantibodies, IAA ELISA KIT has been pre-coated with an Insulin Autoantibodies(IAA antibody specific to Insulin Autoantibodies(IAA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Insulin Autoantibodies(IAA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Insulin Autoantibodies(IAA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Insulin Autoantibodies(IAA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Animal Models of Insulin-Dependent Diabetes
with the development of insulin autoantibodies and insulitis. Accurate measurement of antiislet autoantibodies by radioassay and detection of antigen-specific T cells using major histocompatibility complex tetramers are possible. Various strategies have been developed
PriCells: Introduction of Isolation and Culture of Human Alveolar Epithelial Cells
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and, vice versa, insulin receptor crossreacts with IGF-1. Numerous studies suggest that IGF-1-R is very important for mitogenesis and is essential for phenotype transformation, at least in rodents (1). In particular, the IGF-1-R has been described in human breast
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