- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-04088h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Neural cell adhesion molecule 2, NCAM2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Neural cell adhesion molecule 2, NCAM2 ELISA KIT
Product Name:Human Neural cell adhesion molecule 2, NCAM2 ELISA KIT
Packing:96T
Catalog No.:ELI-04088h
Gene Name:NCAM2
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Neural cell adhesion molecule 2(NCAM2
Alternative Name:NCAM2,Neural cell adhesion molecule 2(NCAM2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Neural cell adhesion molecule 2, NCAM2 ELISA KIT allows for the in vitro quantitative determination of Neural cell adhesion molecule 2(NCAM2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Neural cell adhesion molecule 2, NCAM2 ELISA KIT has been pre-coated with an Neural cell adhesion molecule 2(NCAM2 antibody specific to Neural cell adhesion molecule 2(NCAM2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Neural cell adhesion molecule 2(NCAM2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neural cell adhesion molecule 2(NCAM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Neural cell adhesion molecule 2(NCAM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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N-CAM是膜糖蛋白,至少以三种形式存在,但都是由同一基因编码。其中两种是跨膜蛋白,第三种共价结合在细胞质膜的外表面。无论是何种存在方式,N-CAM分子都有一部分伸出到细胞外表面。三种N-CAM在细胞外的结构都一样,都含有与细胞粘着相关的结合位点。 将分离纯化的N-CAM加入到人工磷脂脂质体中, 这种人工脂质体能够相互粘着,但是加入了相应的抗体就不会发生粘着, 不加N-CAM的人工脂质体也不会发生粘着。由此可以推测:细胞粘着是由细胞质膜中的蛋白分子介导的,N
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