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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
CAMPATH- 1 antigen, CD52
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human CAMPATH- 1 antigen, CD52 ELISA KIT
Product Name:Human CAMPATH- 1 antigen, CD52 ELISA KIT
Packing:96T
Catalog No.:ELI-03729h
Gene Name:CD52
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:CAMPATH-1 antigen(CD52
Alternative Name:CD52,CAMPATH-1 antigen(CD52
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human CAMPATH- 1 antigen, CD52 ELISA KIT allows for the in vitro quantitative determination of CAMPATH-1 antigen(CD52 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human CAMPATH- 1 antigen, CD52 ELISA KIT has been pre-coated with an CAMPATH-1 antigen(CD52 antibody specific to CAMPATH-1 antigen(CD52 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CAMPATH-1 antigen(CD52 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CAMPATH-1 antigen(CD52, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of CAMPATH-1 antigen(CD52 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验CD52 分子 常用单克隆抗体或代号: YTH66.9;(Campath- 1) 主要表达细胞: Leu,Eo [NL] 分子质量(kDa)和结构: gp25- 29 功 能: 补体介导溶解作用的靶分子 Aka Campath-1 CD52 antibodies are lytic for target cells, both with human complement and via antibody dependent cellular
consists of a cooperation between monocytes and cytophilic antibodies able to bind to the Fc receptors present on the monocyte surface. Thus human IgG1 and IgG3 are the main isotypes effective in ADCI, whereas IgG2, IgG4, and IgM are ineffective
Ultrasensitive ELISA for Measurement of Human Cytokine Responses in Primary Culture
ELISAs offer excellent specificity and, once fully optimized, sensitivity that rivals that of bioassays. The major variables that need to be experimentally determined when developing an ELISA are the optimal number of fresh cells required
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