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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Alpha- fetoprotein Lens culinaris agglutiin 3, AFP-L3 ELIS
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Alpha-fetoprotein Lens culinaris agglutiin 3, AFP- L3 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
AFPL3,Alpha-fetoprotein Lens culinaris agglutiin 3, AFP-L3; Alpha-Fetoprotein Lens culinaris agglutiin 3; Alpha-fetoprotein isoform; AFP-L3
Search name
Human AFPL3 ELISA KIT ,Human Alpha-fetoprotein Lens culinaris agglutiin 3 ELISA KIT ,Human AFP-L3 ELISA KIT ,Human Alpha-Fetoprotein Lens culinaris agglutiin 3 ELISA KIT ,Human Alpha-fetoprotein isoform ELISA KIT ,Human AFP-L3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human Alpha-fetoprotein Lens culinaris agglutiin 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-fetoprotein Lens culinaris agglutiin 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-fetoprotein Lens culinaris agglutiin 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-fetoprotein Lens culinaris agglutiin 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-fetoprotein Lens culinaris agglutiin 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验cytotoxic cell 抗体依赖细胞毒细胞 ADH antidiuretic hormone 抗利尿激素 AEC 3-amino-9-ethyl-carbazole 3-氨-9-乙基咔唑 AFP alpha(α)-fetal-protein 甲胎蛋白 AHA anti-histone antibody 抗组蛋白抗体 AKP alkaline phosphatase 碱性磷酸酶 ALP Alkaline Phosphatase 碱性
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