- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-03624h
- 2025年07月10日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Myosin light chain kinase, smooth muscle, MYLK
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Myosin light chain kinase, smooth muscle, MYLK ELISA KIT
Product Name:Human Myosin light chain kinase, smooth muscle, MYLK ELISA KIT
Packing:96T
Catalog No.:ELI-03624h
Gene Name:MYLK
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Myosin light chain kinase(smooth muscle(MYLK
Alternative Name:MYLK,Myosin light chain kinase(smooth muscle(MYLK
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Myosin light chain kinase, smooth muscle, MYLK ELISA KIT allows for the in vitro quantitative determination of Myosin light chain kinase(smooth muscle(MYLK concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Myosin light chain kinase, smooth muscle, MYLK ELISA KIT has been pre-coated with an Myosin light chain kinase(smooth muscle(MYLK antibody specific to Myosin light chain kinase(smooth muscle(MYLK .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Myosin light chain kinase(smooth muscle(MYLK and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Myosin light chain kinase(smooth muscle(MYLK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Myosin light chain kinase(smooth muscle(MYLK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Purification of Myosin Light Chain Kinase
RPM (4℃).Filter the supernatant through glass wool and discard the pellet (or save for light chain prep).Save 1 ml of supernatant (4℃)for assay.Add pepstatin and TLCK to final concentrations of 1 μg/ml and 40 Og/ml,respectively. Volume of supernatant
Myosin Light Chain Preparation
in lyophilizer bottles and freeze in liquid nitrogen.Put on lyophilizer and leave until dry (overnight or over weekend). Day 5 22.Resuspend separate light chain pools 1 and 2 in a small volume of Dialyzing Solution G.Pipette into dialyzing tubing (size #20
Isolation and Culture of Human Airway Smooth Muscle Cells
doublings and express smooth muscle cell-specific markers such as myosin heavy chain, myosin light chain kinase, α-actin, caldesmon, and SM22α . In addition, the synthetic function of these cells appears to be comparable to those studied in vivo
技术资料暂无技术资料 索取技术资料
