- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-03548h
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Prolyl 4- hydroxylase subunit alpha- 1, P4HA1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Prolyl 4- hydroxylase subunit alpha- 1, P4HA1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
P4HA1,P4HA; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline, 2-oxoglutarate 4-dioxygenase ,proline 4-hydroxylase alpha polypeptide I; procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; prolyl 4-hydroxylase alpha polypeptide I; prolyl 4-hydroxylase subunit alpha 1, Prolyl 4-hydroxylase subunit alpha-1,4-PH alpha-1
Search name
Human P4HA1 ELISA KIT ,Human P4HA ELISA KIT ,Human C-P4Halpha(I) ELISA KIT ,Human collagen prolyl 4-hydroxylase alpha(I) ELISA KIT ,Human procollagen-proline ELISA KIT ,Human 2-oxoglutarate 4-dioxygenase ELISA KIT ,Human proline 4-hydroxylase alpha polypeptide I ELISA KIT ,Human procollagen-proline ELISA KIT ,Human 2-oxoglutarate-4-dioxygenase subunit alpha-1 ELISA KIT ,Human prolyl 4-hydroxylase alpha polypeptide I ELISA KIT ,Human prolyl 4-hydroxylase subunit alpha 1 ELISA KIT ,Human Prolyl 4-hydroxylase subunit alpha-1 ELISA KIT ,Human 4-PH alpha-1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human 4-PH alpha-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to 4-PH alpha-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for 4-PH alpha-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 4-PH alpha-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 4-PH alpha-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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