- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-03455h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Ubiquitin- conjugating enzyme E2 B, UBE2B
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Ubiquitin- conjugating enzyme E2 B, UBE2B ELISA KIT
Product Name:Human Ubiquitin- conjugating enzyme E2 B, UBE2B ELISA KIT
Packing:96T
Catalog No.:ELI-03455h
Gene Name:UBE2B
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Ubiquitin-conjugating enzyme E2 B(UBE2B
Alternative Name:UBE2B,Ubiquitin-conjugating enzyme E2 B(UBE2B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ubiquitin- conjugating enzyme E2 B, UBE2B ELISA KIT allows for the in vitro quantitative determination of Ubiquitin-conjugating enzyme E2 B(UBE2B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ubiquitin- conjugating enzyme E2 B, UBE2B ELISA KIT has been pre-coated with an Ubiquitin-conjugating enzyme E2 B(UBE2B antibody specific to Ubiquitin-conjugating enzyme E2 B(UBE2B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ubiquitin-conjugating enzyme E2 B(UBE2B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ubiquitin-conjugating enzyme E2 B(UBE2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ubiquitin-conjugating enzyme E2 B(UBE2B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
in Insect Cells Using a Baculovirus System Alternate Protocol 11: Preparation of Rabbit E1 Support Protocol 4: Generation of Empty Bacterial Lysates Generation of Ubiquitin‐Conjugating Enzymes (E2s) Basic Protocol 22: Expression of E2s in E. coli
Human IL-2 Enzyme Immunoassay (EIA)
-2 EIA is an in vitro enzyme-linked immunosorbent assay for quantitatively detecting human IL-2 in biological fluids (e.g. serum, plasma) and in cell culture supernatants. The kit consists of a 96 well microtiter plate that has been precoated with anti-IL
ELISPOT (Enzyme-linked ImmunoSPOT)
Introduction Enzyme-linked immunosorbent spot (ELISPOT) assays were originally developed to enumerate B cells secreting antigen-specific antibodies, but subsequently the assay has been adapted for identification and enumeration
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