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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Serum albumin, ALB
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Serum albumin, SA ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Serum albumin, SA,albumin,serum albumin; PRO0883; PRO0903; PRO1341; 32 AA; albumin AA 34; cell growth inhibiting protein 42; growth-inhibiting protein 20,BSA
Search name
Human Serum albumin ELISA KIT ,Human SA ELISA KIT ,Human albumin ELISA KIT ,Human serum albumin ELISA KIT ,Human PRO0883 ELISA KIT ,Human PRO0903 ELISA KIT ,Human PRO1341 ELISA KIT ,Human 32 AA ELISA KIT ,Human albumin AA 34 ELISA KIT ,Human cell growth inhibiting protein 42 ELISA KIT ,Human growth-inhibiting protein 20 ELISA KIT ,Human BSA ELISA KIT, Human ALB ELISA KIT
Intended use
Human Serum albumin ELISA KIT allows for the in vitro quantitative determination of human serum albumin,Serum albumin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Human Serum albumin ELISA KIT has been pre-coated with an antibody specific to Serum albumin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Serum albumin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Serum albumin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Serum albumin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Serum albumin ELISA KIT Human-Serum-albumin-SA-ELISA-Kit.pdf">PDF Manual download
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文献和实验Screening Major Binding Sites on Human Serum Albumin by Affinity Capillary Electrophoresis
A screening method is described for determining whether a drug or small solute has significant interactions at the two major binding sites on human serum albumin (HSA). This method uses affinity capillary electrophoresis (ACE) to perform
Serum is a readily available source for diagnostic assays, but the identification of disease-specific serum biomarkers has been impeded by the dominance of human serum albumin (HSA) and immunoglobulin G (IgG) in the serum proteome
of these tissues would be assisted if contaminating serum albumin could be reduced. This chapter outlines a protocol for the effective reduction of serum albumin levels from human myocardium extracts enriched for soluble cytoplasmic proteins.
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