- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-03121h
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Cross- linked C- terminal telopeptide of Collagen alpha- 1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Cross-linked C-terminal telopeptide of Collagen alpha-1(I) chain, ICTP ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Cross-linked C-terminal telopeptide of Collagen alpha-1(I) chain, ICTP, Carboxy-terminal telopepide type I collagen
Search name
Human ICTP ELISA Kit, Human Cross-linked C-terminal telopeptide of Collagen alpha-1(I) chain ELISA Kit, Human Carboxy-terminal telopepide type I collagen ELISA Kit, ICTP ELISA Kit, Human Cross-linked C-terminal telopeptide of Collagen alpha-1(I) chain ELISA Kit, Carboxy-terminal telopepide type I collagen ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human Cross-linked C-terminal telopeptide of Collagen alpha-1(I) chain,ICTP concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
AnchorTest principle
AnchorThe ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ICTP, During the reaction, ICTP in the sample or standard competes with a fixed amount of biotin-labeled ICTP for sites on a pre-coated Monoclonal antibody specific to ICTP. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ICTP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验the supernatant, and re-dissolve the precipitate in 0.25 M acetic acid at ˜ 1:1 ratio by stirring for 24 h at 4°C. 9) Dialyze the collagen solution against 6-8 changes of 6L ˜17.5 mM acetic acid (1 ml glacial acetic acid per liter of cold water
under eyelid Chédiak-Higashi syndrome Linked to consanguinity and transmitted as an autosomal recessive trait
CD49a/CD49b/CD49c/CD49d/CD49e/CD49f分子
, fibronectin, e-cadherin Responsible for platelet adhesion to collagen (blood clotting), role in angiogenesis Positive staining (normal): platelets, activated B & T cells CD49c Aka very late antigen (VLA) alpha 3 chain
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