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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Cellular tumor antigen p53, TP53
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Cellular tumor antigen p53, TP53 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Cellular tumor antigen p53,Tumor suppressor p53,TP53,p53,BCC7; LFS1; TRP53; antigen NY-CO-13; cellular tumor antigen p53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; tumor protein p53
Search name
Human Cellular tumor antigen p53 ELISA KIT ,Human Tumor suppressor p53 ELISA KIT ,Human TP53 ELISA KIT ,Human p53 ELISA KIT ,Human BCC7 ELISA KIT ,Human LFS1 ELISA KIT ,Human TRP53 ELISA KIT ,Human antigen NY-CO-13 ELISA KIT ,Human cellular tumor antigen p53 ELISA KIT ,Human p53 tumor suppressor ELISA KIT ,Human phosphoprotein p53 ELISA KIT ,Human transformation-related protein 53 ELISA KIT ,Human tumor protein p53 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human Cellular tumor antigen p53 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cellular tumor antigen p53. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cellular tumor antigen p53 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cellular tumor antigen p53, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cellular tumor antigen p53 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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