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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Glycogen phosphorylase, liver form, PYGL
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Glycogen phosphorylase, liver form, PYGL ELISA KIT
Product Name:Human Glycogen phosphorylase, liver form, PYGL ELISA KIT
Packing:96T
Catalog No.:ELI-02891h
Gene Name:PYGL
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Glycogen phosphorylase(liver form(PYGL
Alternative Name:PYGL,Glycogen phosphorylase(liver form(PYGL
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Glycogen phosphorylase, liver form, PYGL ELISA KIT allows for the in vitro quantitative determination of Glycogen phosphorylase(liver form(PYGL concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Glycogen phosphorylase, liver form, PYGL ELISA KIT has been pre-coated with an Glycogen phosphorylase(liver form(PYGL antibody specific to Glycogen phosphorylase(liver form(PYGL .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Glycogen phosphorylase(liver form(PYGL and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glycogen phosphorylase(liver form(PYGL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Glycogen phosphorylase(liver form(PYGL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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途径产生 NADPH,NADPH 维持细胞内氧化还原稳态,保证 Tm 细胞二次激活反应时的高质量和高活性。 糖原磷酸化酶(Glycogen phosphorylase,PYG)是糖原分解反应的关键酶,催化糖原裂解为 1 磷酸葡萄糖,具有三种亚型(PYGL,Liver;PYGM, Muscle;PYGB, Brain)。本研究发现 PYGB 在 T 细胞中高表达。 重要的是,PYG 一直被认为通过其丝氨酸磷酸化而发挥活性功能;本研究还首次发现 TCR 的下游信号分子 ZAP70/LCK 能够
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