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Human Glycogen phosphorylase,b

rain form,PYGB ELISA KIT
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  • ¥2890
  • LSM Bio
  • 0.15-10ng/ml
  • 0.078ng/ml
  • 国产
  • ELI-02875h
  • 2025年07月10日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      50

    • 供应商

      LSMBIO

    • 检测范围

      0.15-10ng/ml

    • 检测方法

      夹心法/竞争法

    • 应用

      夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度

    • 适应物种

    • 标记物

      Glycogen phosphorylase, brain form, PYGB

    • 样本

      人血清、血浆或其它相关生物液体中天然和重组蛋白

    • 灵敏度

      0.078ng/ml

    • 规格

      96T

    Human Glycogen phosphorylase, brain form, PYGB ELISA KIT

    96 Tests

    Operating instruction

     

    FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

     

    Synonyms

    PYGB,GPBB; glycogen phosphorylase B; phosphorylase glycogen brain, Glycogen phosphorylase brain form

     

    Search name

    Human PYGB ELISA KIT ,Human GPBB ELISA KIT ,Human glycogen phosphorylase B ELISA KIT ,Human phosphorylase glycogen brain ELISA KIT ,Human Glycogen phosphorylase brain form ELISA KIT ,Human Glycogen phosphorylase brain ELISA KIT

     

    Intended use

    This immunoassay kit allows for the in vitro quantitative determination of human Glycogen phosphorylase, brain form concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

     

    Test principle

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycogen phosphorylase, brain form. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Glycogen phosphorylase, brain form and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glycogen phosphorylase, brain form, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glycogen phosphorylase, brain form in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    PDF Manual download
     

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    图标文献和实验
    该产品被引用文献
    "Pioglitazone Enhances β-Arrestin2 Signaling and Ameliorates Insulin Resistance in Classical Insulin Target Tissues Authors: El-Fayoumi S. ·Mansour R. · Mahmoud A · Fahmy A. · Ibrahim I. Pharmacology 2021, Vol.106, No. 7-8 July 2021Pioglitazone Enhances β-Arrestin2 Signaling and Ameliorates Insulin Resistance in Classical Insulin Target Tissues Authors: El-Fayoumi S. ·Mansour R. · Mahmoud A · Fahmy A. · Ibrahim I. Pharmacology 2021, Vol.106, No. 7-8 July 2021"
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      途径产生 NADPH,NADPH 维持细胞内氧化还原稳态,保证 Tm 细胞二次激活反应时的高质量和高活性。 糖原磷酸化酶(Glycogen phosphorylase,PYG)是糖原分解反应的关键酶,催化糖原裂解为 1 磷酸葡萄糖,具有三种亚型(PYGL,Liver;PYGM, Muscle;PYGBBrain)。本研究发现 PYGB 在 T 细胞中高表达。 重要的是,PYG 一直被认为通过其丝氨酸磷酸化而发挥活性功能;本研究还首次发现 TCR 的下游信号分子 ZAP70/LCK 能够

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    文献支持
    Human Glycogen phosphorylase,brain form,PYGB ELISA KIT
    ¥2890