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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Glycogen phosphorylase, brain form, PYGB
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Glycogen phosphorylase, brain form, PYGB ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PYGB,GPBB; glycogen phosphorylase B; phosphorylase glycogen brain, Glycogen phosphorylase brain form
Search name
Human PYGB ELISA KIT ,Human GPBB ELISA KIT ,Human glycogen phosphorylase B ELISA KIT ,Human phosphorylase glycogen brain ELISA KIT ,Human Glycogen phosphorylase brain form ELISA KIT ,Human Glycogen phosphorylase brain ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human Glycogen phosphorylase, brain form concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycogen phosphorylase, brain form. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Glycogen phosphorylase, brain form and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glycogen phosphorylase, brain form, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Glycogen phosphorylase, brain form in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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