- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-02707h
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Hypoxia- inducible factor 1- alpha, HIF1A
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Hypoxia- inducible factor 1- alpha, HIF1A ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
HIF1A,HIF-1 alpha,HIF-1-alpha,HIF-1A,HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78,Hypoxia-inducible factor 1-alpha,HIF1-alpha,ARNT interacting protein,ARNT-interacting protein,PAS domain-containing protein 8,basic-helix-loop-helix-PAS protein MOP1,class E basic helix-loop-helix protein 78,hypoxia inducible factor 1 alpha subunit,basic helix-loop-helix transcription factor,hypoxia-inducible factor 1 alpha isoform I.3,hypoxia-inducible factor 1 alpha subunit,basic helix-loop-helix transcription factor,hypoxia-inducible factor1alpha,member of PAS protein 1,member of PAS superfamily 1,hypoxia inducible factor 1 alpha subunit
Search name
Human HIF1A ELISA KIT ,Human HIF-1 alpha ELISA KIT ,Human HIF-1-alpha ELISA KIT ,Human HIF-1A ELISA KIT ,Human HIF-1alpha ELISA KIT ,Human HIF1 ELISA KIT ,Human HIF1-ALPHA ELISA KIT ,Human MOP1 ELISA KIT ,Human PASD8 ELISA KIT ,Human bHLHe78 ELISA KIT ,Human Hypoxia-inducible factor 1-alpha ELISA KIT ,Human HIF1-alpha ELISA KIT ,Human ARNT interacting protein ELISA KIT ,Human ARNT-interacting protein ELISA KIT ,Human PAS domain-containing protein 8 ELISA KIT ,Human basic-helix-loop-helix-PAS protein MOP1 ELISA KIT ,Human class E basic helix-loop-helix protein 78 ELISA KIT ,Human hypoxia inducible factor 1 alpha subunit ELISA KIT ,Human basic helix-loop-helix transcription factor ELISA KIT ,Human hypoxia-inducible factor 1 alpha isoform I.3 ELISA KIT ,Human hypoxia-inducible factor 1 alpha subunit ELISA KIT ,Human basic helix-loop-helix transcription factor ELISA KIT ,Human hypoxia-inducible factor1alpha ELISA KIT ,Human member of PAS protein 1 ELISA KIT ,Human member of PAS superfamily 1 ELISA KIT ,Human hypoxia inducible factor 1 alpha subunit ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human hypoxia-inducible factor 1-alpha,HIF-1-alpha concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HIF-1-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HIF-1-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HIF-1-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HIF-1-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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