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细胞污染识别与处理全攻略:5 种常见类型+关键误区Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Glycogen synthase kinase- 3 alpha, GSK3A
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Glycogen synthase kinase- 3 alpha, GSK3A ELISA KIT
Product Name:Human Glycogen synthase kinase- 3 alpha, GSK3A ELISA KIT
Packing:96T
Catalog No.:ELI-02620h
Gene Name:GSK3A
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Glycogen synthase kinase-3 alpha(GSK3A
Alternative Name:GSK3A,Glycogen synthase kinase-3 alpha(GSK3A
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Glycogen synthase kinase- 3 alpha, GSK3A ELISA KIT allows for the in vitro quantitative determination of Glycogen synthase kinase-3 alpha(GSK3A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Glycogen synthase kinase- 3 alpha, GSK3A ELISA KIT has been pre-coated with an Glycogen synthase kinase-3 alpha(GSK3A antibody specific to Glycogen synthase kinase-3 alpha(GSK3A .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Glycogen synthase kinase-3 alpha(GSK3A and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Glycogen synthase kinase-3 alpha(GSK3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Glycogen synthase kinase-3 alpha(GSK3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Inhibition of Glycogen Synthase Kinase-3 Methods in Molecular Biology v468. chapter 5 Abstract There are two homologous forms of glycogen synthase kinase (GSK)-3, GSK-3a and GSK-3b, whichplay overlapping roles in the regulation of Wnt
Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
and beta isoforms in patients with NIDDM. Diabetologia 40, 940–946. 3. Shaw, P. C., Davies, A. F., Lau, K. F., Garcia-Barcelo, M., Waye, M. M., Lovestone, S., et al. (1998) Isolation and chromosomal mapping of human glycogen synthase kinase-3 alpha
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