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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
C- telopeptide of Collagen alpha- 1(I) chain, CTXI ELISA K
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human C- telopeptide of Collagen alpha- 1(I) chain, CTXI ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Collagen alpha-1(I) chain,Alpha-1 type I collagen, CTXI, C- telopeptide of Collagen alpha- 1(I) chain
Search name
Human CTXI ELISA Kit, Human C- telopeptide of Collagen alpha- 1(I) chain ELISA Kit, Human Collagen alpha-1(I) chain ELISA Kit, Human Alpha-1 type I collagen ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human collagen alpha-1(i) chain,Collagen alpha-1(I) chain concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen alpha-1(I) chain. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Collagen alpha-1(I) chain and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Collagen alpha-1(I) chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Collagen alpha-1(I) chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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