- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELA-E9990h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Carbonic Anhydrase IV
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Carbonic Anhydrase IV ELISA Kit
Product Name:Human Carbonic Anhydrase IV ELISA Kit
Packing:96T
Catalog No.:ELA-E9990h
Gene Name:CA4
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Carbonic Anhydrase IV
Alternative Name:CA4,Carbonic Anhydrase IV
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Carbonic Anhydrase IV ELISA Kit allows for the in vitro quantitative determination of Carbonic Anhydrase IV concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Carbonic Anhydrase IV ELISA Kit has been pre-coated with an Carbonic Anhydrase IV antibody specific to Carbonic Anhydrase IV .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Carbonic Anhydrase IV and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Carbonic Anhydrase IV, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Carbonic Anhydrase IV in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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【Jounal club】NATURE MEDICINE: 利用蛋白组技术发现糖尿病眼底病变新机制
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The Application of the Chemical Array for Biological Study
library GLORIA (Gene Library of Osada Laboratory at RIKEN for chemical array analysis) has been established. Using the systematic platform, we detected novel inhibitors of carbonic anhydrase II. We also have shown that this screening method is useful
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