Human Nicotinamide Adenine Din

【STORAGE】

【PRINCIPLE OF THE ASSAY】

This assay employs a two-site sandwich ELISA to quantitate NOX4 in Human serum, plasma. An antibody specific for NOX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NOX4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOX4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOX4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

【LIMITATIONS OF THE PROCEDURE】

※ FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

※ The kit should not be used beyond the expiration date on the kit label.

※ Do not mix or substitute reagents with those from other lots or sources.

※ It is important that the Calibrator Diluent selected for the standard curve be

consistent with the samples being assayed.

※       If samples generate values higher than the highest standard, dilute the samples with the appropriate Calibrator Diluent and repeat the assay.

※       Any variation in standard diluent, operator, pipetting technique, washing

technique, incubation time or temperature, and kit age can cause variation in binding.

※       This assay is designed to eliminate interference by soluble receptors,

binding proteins, and other factors present in biological samples. Until all factors have been tested in the ELISA Kit, the possibility of interference cannot be excluded.

【DETECTION RANGE】

31.25 pg/mL - 2000 pg/mL. The standard curve concentrations used for the ELISA’s were 2000 pg/mL, 1000 pg/mL, 500 pg/mL, 250 pg/mL, 125 pg/mL, 62.5 pg/mL,
31.25 pg/mL, 0 pg/mL.

【SENSITIVITY】

The limit of detection of Human NOX4 defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 15.6 pg/mL (mean of 6 independent assays).

【SPECIFICITY】

This assay has high sensitivity and excellent specificity for detection of Human NOX4. No significant cross-reactivity or interference between Human NOX4 and analogues was observed.

Note:

Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between Human NOX4 and all the analogues, therefore, cross reaction may still exist.

【PRECISION】

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8%
Inter-Assay: CV<12%