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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Protein Kinase C Beta 2
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Protein Kinase C Beta 2 ELISA Kit
Product Name:Human Protein Kinase C Beta 2 ELISA Kit
Packing:96T
Catalog No.:ELA-E9429h
Gene Name:PKCBeta2
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Protein Kinase C Beta 2
Alternative Name:PKCBeta2,Protein Kinase C Beta 2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Protein Kinase C Beta 2 ELISA Kit allows for the in vitro quantitative determination of Protein Kinase C Beta 2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Protein Kinase C Beta 2 ELISA Kit has been pre-coated with an Protein Kinase C Beta 2 antibody specific to Protein Kinase C Beta 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Protein Kinase C Beta 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein Kinase C Beta 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Protein Kinase C Beta 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Translocation Assays of Protein Kinase C Activation
The protein kinase C (PKC) family members include at least 11 different isoforms that, based on their different requirements for activation, have been divided into three subfamilies, the Ca2+ -dependent (cPKCα, (βI , βII
Protein Kinase C as an Effector of Lipid-Derived Second Messengers
the activation and function of protein kinase C in these immune cells. Since neutrophils provide a ready source of human primary tissue, these methods are also useful for pharmacological studies on the protein kinase C system and for evaluation of protein kinase
Monitoring Protein Kinase A Activities Using Expressed Substrate in Live Cells
Protein kinase A (PKA) activity is regulated by intracellular cyclic adenosine mono-phosphate. Conventional protein kinase assays after cell lysis are hence not suitable for analyzing PKA activities. In this chapter, we describe a new method
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