- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELI-02043h
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
N- terminal propeptide of Collagen alpha- 1(III) chain, PI
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human N-terminal propeptide of Collagen alpha-1(III) chain, PIIINP ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
N-terminal propeptide of Collagen alpha-1(III) chain,PIIINP, N- terminal procollagen III propeptide
Search name
Human PIIINP ELISA Kit, Human N-terminal propeptide of Collagen alpha-1(III) chain ELISA Kit, Human N- terminal procollagen III propeptide ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human N-terminal propeptide of Collagen alpha-1(III) chain concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-terminal propeptide of Collagen alpha-1(III) chain, During the reaction, N-terminal propeptide of Collagen alpha-1(III) chain in the sample or standard competes with a fixed amount of biotin-labeled N-terminal propeptide of Collagen alpha-1(III) chain for sites on a pre-coated Monoclonal antibody specific to N-terminal propeptide of Collagen alpha-1(III) chain. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of N-terminal propeptide of Collagen alpha-1(III) chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Type II collagen, the most abundant protein of cartilage matrix, is synthesized as a procollagen molecule including the N-(PIINP) and C-(PIICP) propeptides at each end. Type II procollagen is produced in two forms as the result of alternative
Introduction of human 14-3-3 proteins
for the C terminus of epsilon isoform,protease digestion of intact 14-3-3 showed that the N-terminal half of 14-3-3 (a 16 kDa fragment)was an intact,dimeric domain of the protein.Two isoforms of 14-3-3,tau and epsilon,were expressed in E.coli
/j.1365-2133.2010.09699.x BJD9699 [pii] (2010). 7. Fusenig, N.E., et al . Growth and differentiation characteristics of transformed keratinocytes from mouse and human skin in vitro and in vivo . J. Invest. Dermatol. 81, 168s-175s (1983
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